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KMID : 0371320040660010001
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Journal of the Korean Surgical Society
2004 Volume.66 No. 1 p.1 ~ p.4
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A Study for Apoptosis and Its Mechanism of Allogeneic Activated T Lymphocytes Induced by Mouse Liver Immature Dendritic Cells
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Lee Young-Cheol
Kim Jeong-Jin
Park Hye-Rim
Lee Sam-Uel
Woo Young-Min
Cho Ma-Hae
Kim Joo-Seop
Lee Bong-Wha
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Abstract
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Purpose: Mouse liver nonparenchymal cells play an important role n the development of active apoptosis in graftinfiltrating cytotoxic T lymphocytes, and this apoptosis can be an explanation for liver graft acceptance. We intended to clarify whether immature mouse liver dendritic cells can induce apoptosis in allogeneic activated T cells and determine which mechanism is involved in this phenomenon.
Methods: A radiometric DNA fragmentation test ("JAM" assay) was used to determine whether mouse liver dendritic cells were eble to induce activated T-cell apoptosis in vitro. In addition, immunohistochemical staining for Bax and Bcl-2 was examined to clarify whether Bax or Bcl-2 was involved in this apoptosis.
Results: Immature mouse liver dendritic cells were quite strong inducers of activated T cell apoptotic death in allogeneic mice in vitro (39.2±13.2% at E/T ratio=12.5/1) compared with spleen cells as effectors (4.7±13.4% at E/T ratio=12.5/1) (P<0.0001). By using immunohistochemical staining, we also showed that Bax might play some role in this phenonenon, but that Bcl-2 might not.
Conclusion: Our data indicate that immature mouse liver dendritic cells might have a strong apoptotic activity toward activated T cells in allogeneic mice in vitro through a Bax involved mechanism.
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